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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has been observed in many tumors, which results in chromosome instability, and so may possibly lead to tumorigenesis. This protein was discovered to affiliate with, and be associated with, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in cell-cycle controlled histone gene expression and performs a essential part in advertising mobile-cycle development while in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are already described. Two more splice variants had been claimed but specific nucleotide sequence details is not really nevertheless accessible.
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Blocking peptides are peptides that bind specially towards the focus on antibody and block antibody binding. These peptide usually includes the epitope regarded by the antibody.
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This manual outlines further probable optimization of mobile and immunoassay parameters to make certain the best possible results are...
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This tutorial outlines even further probable optimization of mobile and immunoassay parameters to be certain the very best results are...
Antibodies bound to the blocking peptide no longer bind to the epitope on the concentrate on protein. By evaluating the staining with the blocked antibody compared to the antibody alone, one can see which staining is unique.
Concentrate on Information and facts Cyclin E belongs to your remarkably conserved cyclin household, whose customers are characterized by a dramatic periodicity in protein abundance in the mobile cycle. Cyclins operate as regulators of CDK kinases. Distinct cyclins exhibit distinctive expression and degradation designs which contribute to the temporal coordination of every mitotic function. Cyclin E kinds a fancy with and capabilities for a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates within the G1-S period boundary and it is degraded as cells development by way of S phase.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both equally antibodies...
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